CRISP-R/Cas9 Mediated Deletion of Copper Transport Genes CTR1 and DMT1 in NSCLC Cell Line H1299. Biological and Pharmacological Consequences

Copper, the highly toxic micronutrient, plays two essential roles: it is a catalytic and structural cofactor for Cu-dependent enzymes, and it acts as a secondary messenger.In the cells, copper is imported by CTR1 (high-affinity copper transporter 1), a transmembrane high-affinity copper importer, and DMT1 (divalent metal transporter).In cytosol, en

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Complex Technique of Large Sural Flap: An Alternative Option for Free Flap in Large Defect of the Traumatized Foot

The distally based sural fasciocutaneous flap has become a main part of the reconstruction of the lower leg, heel and foot.However, perfusion problems and venous congestion have been reported.Over the past decade, several flap modifications have been reported to improve flap viability and to solve a myriad of reconstructive needs.The purpose of thi

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The Borax Method of Gold Extraction for Small-Scale Miners

Background.More than ten million small-scale miners (SSM) worldwide use mercury to extract gold, releasing large amounts of the toxic element into the environment.Alternatives to mercury have been suggested over the years to little avail.A group of miners in the Philippines has demonstrated that borax, when used as a flux for smelting gold out of h

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